WebThis promoter is critical in that it determines the time and site of Cre expression. Cre enzyme, in turn, recombines the floxed gene and produces gene knockout. Here we review Cre mouse lines that have been developed to target either the entire brain, selected brain areas, or specific neuronal populations. WebThe Cre mouse line with progesterone receptor promoter ( PgrCre) has been widely used …
Generation and characterization of CD19-iCre mice as a tool for ...
WebResults showed that the HIF1A-As2 promoter region was significantly enriched in MYC antibody-bound complexes compared to IgG. ... C Representative images of high-power magnifications of lungs harvested from KRAS LSLG12D mice treated with CRE +/ASO Ctrl or CRE +/ASO#1. Scale bar, 1 mm. Lung parenchyma affected by several epithelial lesions is ... WebJan 1, 2014 · Many promoter-Cre constructs contain a reporter gene, Gfp, downstream of Cre. Thus, the expression of the Gfp gene can be used to show where the promoter was expressed. By looking earlier in gestation, it is possible to determine when promoter expression first occurs. It is at this time point that Cre transcription would be anticipated … ny train derailment
Endothelial-Specific Cre Mouse Models Arteriosclerosis, …
WebThe Cre mouse line with progesterone receptor promoter ( PgrCre) has been widely used to produce specific uterine gene-deficient mice, but in the Cre line, endogenous Pgr gene is replaced by Cre recombinase gene, which makes the breeding of homozygous mice ( PgrCre/Cre) difficult because they are infertile. Web162387. pGP-20XUAS-IVS-Syn21-jGCaMP8m-p10. Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster. Loading... 162386. pGP-20XUAS-IVS-Syn21-jGCaMP8s-p10. Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster. WebSep 17, 2024 · The Cre/Esr1 is constitutively expressed, but only translocates to the nucleus when bound to TAM’s metabolite 4-OHT where it then recombines LoxP sites. In the case of this YFP reporter, the... ny trail maps