Primescript double strand cdna synthesis kit
WebApr 13, 2024 · RNAiso reagent (TaKaRa, Japan) was used to extract the total cellular RNA, and PrimeScript™ RT kit (TaKaRa, Japan) was used to obtain the cDNA. SYBR Premix Ex Taq II real-time PCR kit (TaKaRa, JAPAN) was used to detect the mRNA expression of the gene. GAPDH was set as the internal control. For the miRNAs, Mir-X™ miRNA first-strand … WebDrought stress is one of the major contributing that interested corn yield. Glyceraldehyde-3-Phosphate dehydrogenase (GAPDH) will an multi-purpose enzyme that plays one important role in abiotic load and plant development. However, for wheat, narrow information regarding drought-responsive GAPC genes has been reported, also the mechanism basic the …
Primescript double strand cdna synthesis kit
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WebThe 1st strand of complementary DNA (cDNA) was synthesized with 2 μg RNA by a PrimeScript TM II 1st Strand cDNA Synthesis Kit (TaKaRa, Dalian, China) with oligo d(T) 15. qRT‐PCR was conducted using the SYBR Green FS Universal SYBR Green Master mix (Roche, Cornwall, England, UK) on the CFX96 TM Real‐Time PCR Detection System (Bio‐Rad, … WebThe 1st strand of complementary DNA (cDNA) was synthesized with 2 μg RNA by a PrimeScript TM II 1st Strand cDNA Synthesis Kit (TaKaRa, Dalian, China) with oligo d(T) …
WebThe first-strand of cDNA was synthesized using PrimeScript 1 st Strand cDNA Synthesis Kit (TaKaRa, Japan). PRRSV1 specific probe and primer pairs, ... and then reverse transcribed … WebField Service & Application Specialist, Oneservice GmbH Report this post Report Report
Web8 Products found. QuantiTect Reverse Transcription Kit. For fast cDNA synthesis enabling sensitive real-time two-step RT-PCR for gene expression analysis. miRCURY LNA SYBR ® … WebPrimescript ⅱ 1st Strand Cdna Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, …
WebApr 8, 2024 · Total RNA was isolated from cells using the Fastagen200 kit (Fastagen, Shanghai, China). Reverse transcription of RNA (1.0 μg) to cDNA was conducted by the PrimeScript RT Reagent Kit (TaKaRa, Osaka, Japan). Real-time PCR was carried out using SYBR Premix Ex Taq TM II (TaKaRa, Osaka, Japan) with primers listed in Supplementary …
WebFor reverse transcription, 2 µg of total RNA was used as a template, and the first strand of cDNA was synthesized using a PrimeScript ® 1st Strand cDNA Synthesis Kit (TAKARA, … selwyns coaches fleet listWebThe RNA purity and concentration were measured with a NanoDrop TM ND1000 spectrophotometer (NanoDrop Technologies, Rockland, DE, USA). cDNA was synthesized … selwyns coaches wirralWebLiterature # TM316. The GoScript™ Reverse Transcription System is a convenient kit that includes a reverse transcriptase and an optimized set of reagents for efficient synthesis … selwyns coaches day tripsWebFirst strand cDNA synthesis was performed using the control 1.3 kb poly(A)+ RNA fragment and Oligo(dT) 18 Primer. After second strand synthesis, the resulting double stranded cDNA was analysed on an agarose gel. The kit passes QC requirements if the yield of double stranded cDNA is ≥75 % relative to the starting quantity of mRNA. ≥75 % Conforms selwyns manchesterWebQuantity and Unit. 50 rxn. Unit. Pack. The PrimeScript 1st strand cDNA Synthesis Kit contains all the reagents necessary to synthesize first strand cDNA from total or polyA+ … selwyns coachesWebFeatures of the SuperScript™ IV First-Strand Synthesis System include: • Significantly improved resistance to a variety of inhibitors that can interfere with cDNA synthesis. • … selx2c1z weightselwyns travel runcorn