WebNov 8, 2024 · Cross-linking of PMA with DNA was conducted using PMA-Lite™ LED photolysis device (Biotium, Inc., California, USA). The samples were exposed to blue light for 15 min and gently mixed every 5 min . After the PMA treatment, the samples were washed with PBS solution to wash off residual PMA. Pelleted samples were stored at − 20 °C. WebThe PMA-Lite™ LED Photolysis Device is a light-weight LED light box designed for optimal photolysis of PMAxx™– or PMA-treated samples in viability PCR applications. Each …
PMA-Lite™ LED Photolysis Device - Biotium
WebPMA-Lite™ LED Photolysis Device is specifically designed for photoactivation of propidium monoazide (PMA), PMAxx™, and EMA. It can also function as a general photolysis device … WebThereafter, photoactivation of the mixture was performed for 15 min on a PMA-Lite™ LED Photolysis Device (Biotium) following the manufacturer’s instructions. Total nucleic acid was extracted from the 0.2 mL or 1 mL treated virus concentrates and eluted into 50 µL or 100 µL of nuclease-free water (Promega Corp.), respectively. solving systems of inequalities worksheet pdf
PMA™, PMAxx™, Real-Time PCR Viability Kit, Biotium
WebThe PMA-Lite™ LED Photolysis Device is a light-weight LED light box designed for optimal photolysis of PMAxx™ – or PMA -treated samples in viability PCR applications. Each … WebApr 30, 2024 · PMA-free samples served as controls for each condition tested. Tubes were covered with aluminium foil and shaken on an orbital shaker for 5, 10, 20, 30 or 70 minutes. Samples were then exposed to light using the PMA-Lite LED Photolysis Device (Cambridge BioSciences, Cambridge, UK) for 5, 10, 20, 30 or 40 minutes. WebThe PMA-Lite LED Photolysis Device can be used to provide continuous illumination for up to 18 samples at a time, for convenient and controlled photoactivation of PMA, PMAxx, … solving systems of linear equations kahoot