Fthfs遺伝子
WebNov 15, 2024 · Background Heat shock transcription factor (Hsfs) is widely found in eukaryotes and prokaryotes. Hsfs can not only help organisms resist high temperature, … WebOct 1, 2009 · Based on the analysis of the deduced polypeptide and nucleotide sequences of complete or partial FTHFS or homologous sequences, two new primers were developed for authentic acetogens ().Application of the newly designed primers to PCR, using DNA of target organisms and extracted from environmental samples as template, resulted in a …
Fthfs遺伝子
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WebThe gene-encoding formyltetrahydrofolate synthetase (FTHFS), a key enzyme of this pathway, is highly conserved and can be used as a molecular marker to probe acetogenic communities. However, there is a lack of systematic collection of FTHFS sequence data at nucleotide and protein levels. In an attempt to streamline investigations on acetogens ... WebSep 4, 2024 · The gene-encoding formyltetrahydrofolate synthetase (FTHFS), a key enzyme of this pathway, is highly conserved and can be used as a molecular marker to probe acetogenic communities. However, there ...
WebJun 24, 2024 · In this study, we developed a new high-throughput FTHFS gene sequencing method for acetogenic community profiling and compared it with conventional terminal … WebAcetoScan is a software pipeline for the analysis of Illumina MiSeq sequencing data for the FTHFS (Formyl--tetrahydrofolate synthetase) gene/amplicons. AcetoScan can also process fasta sequences to filter …
Webformyltetrahydrofolate synthetase (FTHFS) gene has been used as an ecological biomarker for reductive acetogenesis [13, 23, 29]. Thus, transcriptional profiling of both the 16S … WebOct 30, 2024 · The abundance of the fthfs gene ranged from 1.92 to 5.40E + 04 copies/ng DNA. It was found that the abundance of the fthfs gene was strongly dependent on the …
WebNov 22, 2011 · Introduction. N 10-Formyltetrahydrofolate synthetase (FTHFS; EC 6.3.4.3) catalyzes the formylation of tetrahydrofolate (THF) in an ATP-dependent manner shown …
Webdetermine the mechanism by which FTHFS oper-ates. It has been suggested that FTHFS employs a random sequential mechanism (random ter ter mechanism)12–14 on the basis of steady-state kinetic data12 and isotope exchange experiments.8,15 How-ever, a more recent, careful kinetic study of FTHFS reports inhibition of the reaction by higher concen- game changers guernseyblackdown pre schoolWebAug 27, 2024 · The formyltetrahydrofolate synthetase (FTHFS) gene is a molecular marker of choice to study the diversity of acetogenic communities. However, current analyses are limited due to lack of a high-throughput sequencing approach for FTHFS gene amplicons and a dedicated bioinformatics pipeline for data analysis, including taxonomic annotation … game changers group home dayton ohioWebJan 24, 2014 · Diversity of FTHFS gene in the gut of giant pandas. A total of 50 FTHFS sequences for each giant panda were subjected to RFLP analysis and sequencing. After HinP1I digestion, three RFLP patterns were found from the sequences libraries of geriatric pandas. However, only one RFLP pattern was found among the cloned sequences from … game changers groupWebAug 16, 2024 · In this study, we applied an acetogen-targeted analyses strategy developed previously by our research group for microbiological surveillance of commercial biogas plants. The surveillance comprised … blackdown qldWebJan 26, 2024 · Acetogens play a key role in anaerobic degradation of organic material and in maintaining biogas process efficiency. Profiling this community and its temporal changes can help evaluate process stability and function, especially under disturbance/stress conditions, and avoid complete process failure. The formyltetrahydrofolate synthetase … gamechangers grand rapisWebMay 11, 2010 · We analysed fragments of the formyltetrahydrofolate synthetase (FTHFS) gene, which encodes a key enzyme in reductive acetogenesis, from the bacterial flora in … game changers golf