WebSep 29, 2024 · Here ID represents the integrated intensity, while AC is the area of selected cells and MFBR is the mean fluorescence of background readings.. 2.7 Induction of chondrogenic differentiation. Chondrogenic differentiation was initiated after a confluent monolayer was formed. The chondrogenic differentiation medium was composed of … WebApr 7, 2024 · Electromechanics of Articular Cartilage. Cartilage is a biphasic tissue, composed of a solid phase, which consists of a charged porous collagen-proteoglycan matrix, and an interstitial fluid phase [ 46 ]. Changes in cartilage composition and arrangement of collagen fibers result in different biomechanical properties.
A New Age for Articular Cartilage: from Bench to Beside of Tissue ...
WebJun 1, 2024 · Moreover, the similar ACAN expression (late chondrogenic marker) on protocols I, II and III clearly point out the need to improve medium composition to induce a more mature differentiation state. The use of a single-differentiation stage (protocol II) resulted on a low number of cells and consequently, lower sGAG production restricted to … WebFinally, culture conditions, chondrogenic medium composition, and the development of novel carriers for controlled delivery of growth factors into scaffolds will also be the focus of this research topic. We will consider articles that explore, but are not limited to: ... they kept on trying their best to develop
Chondrogenic Differentiation - an overview ScienceDirect Topics
WebApr 11, 2024 · Cartilage composition, function, and damage. Cartilage exists in different parts of the body, including joints, ribs, intervertebral discs, ears, ... First, a chondrocyte-hydrogel construct was cultured in chondrogenic medium for 3 weeks for in vitro cartilage regeneration. Then, the construct was implanted into cartilage defects in mice. ... WebThe three media yielded constructs with comparable wet weights and compressive moduli ( approximately 25 kPa). Chondrogenic medium yielded constructs with higher amounts of DNA (1.5-fold) and glycosaminoglycans (GAG, 4 … WebThese MSCs can then effectively differentiate under standard chondrogenic conditions (serum-free medium supplemented with insulin-transferrin-selenite (ITS), ascorbate, proline, dexamethasone, and TGFα3). In addition, MSCs may also be seeded on various three-dimensional scaffolds to induce chondrogenesis. they killed it gif